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Part:BBa_K5152011

Designed by: Paul Lau   Group: iGEM24_HongKong-JSS   (2024-09-26)

3' 20 nt overlapping region for pUC19 EcoRI site

We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using EcoRI and PstI restriction enzymes.

We also designed a 20 nt overlapping region at the 5' end for the PstI site on the pUC19 vector (BBa_K5152010).

We verified that these regions allow for successful and effective assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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